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Image Search Results
Journal: Free radical biology & medicine
Article Title: CaMKII protein expression and phosphorylation in human skeletal muscle by immunoblotting: Isoform specificity.
doi: 10.1016/j.freeradbiomed.2024.08.030
Figure Lengend Snippet: Fig. 1. CaMKII isoforms identification through immunoblotting in human skeletal muscle from a healthy participant obtained under resting conditions. a) Representative image obtained using Image Lab© software 6.0.1 (Bio-Rad) employing Lanes and Bands and MW Analysis Tools. The continuous purple line denotes the selected band used to calculate the molecular weight. Blue vertical lines delimit each lane. b) Representative immunoblot employing five commercial antibodies: Aldrich oxidised-Met281/282, CST pThr287, BD Total, CST Total, Badrilla δ. c) Over-exposed immunoblot targeting βM with the Aldrich oxidised-Met281/
Article Snippet: Experimental molecular weights (MW) of each band resulting from the reactivity with antibodies targeting all total CaMKII and all phosphorylated isoforms, the specific antibodies for the β, γ, δ isoforms, and an antibody specific for oxidised methionine 281/282 were determined using the Lanes and Bands and MW Analysis Tools within the
Techniques: Western Blot, Software, Molecular Weight
Journal: Free radical biology & medicine
Article Title: CaMKII protein expression and phosphorylation in human skeletal muscle by immunoblotting: Isoform specificity.
doi: 10.1016/j.freeradbiomed.2024.08.030
Figure Lengend Snippet: Fig. 2. CaMKII isoforms identification through immunoblotting in human skeletal muscle from a healthy participant obtained under resting conditions. a) Representative image obtained using Image Lab© software 6.0.1 (Bio-Rad) employing Lanes and Bands and MW Analysis Tools. The continuous purple line denotes the selected band used to calculate the molecular weight. Blue vertical lines delimit each lane. b) Representative immunoblot employing five commercial antibodies: BD Total, Abcam δ, Badrilla δ, Proteintech γ and SCBT β. c) Total protein staining using Reactive Brown 10. Isoforms (βM, γ/δ and δD) are depicted on the left side of the panel. The band migration markers indicate the molecular weight in kDa. BD Total: anti-CaMKII total (no. 611292, BD Biosciences); Abcam δ anti-CaMKII delta (no. 181052, Abcam); Badrilla δ anti-CaMKII delta (no. A010-55AP, Badrilla); Proteintech γ: anti-CaMKII Gamma (no. 12666-2-AP, Proteintech); SCBT β: anti-CaMKII Beta (no. 376828, Santa Cruz Biotechnology). Note that the antibody tar geting the γ isoform displays high cross-reactivity with β and δ CaMKII, being unable to specifically resolve the γ isoform.
Article Snippet: Experimental molecular weights (MW) of each band resulting from the reactivity with antibodies targeting all total CaMKII and all phosphorylated isoforms, the specific antibodies for the β, γ, δ isoforms, and an antibody specific for oxidised methionine 281/282 were determined using the Lanes and Bands and MW Analysis Tools within the
Techniques: Western Blot, Software, Molecular Weight, Staining, Migration